Pregnancy Outcomes & Child Development
Epigenetics of Preterm Birth: Preterm birth is a risk factor for child behavior problems and adult psychiatric disorders. We have shown that DNA methylation across the genome associates with gestational age in term deliveries and in spontaneous preterm birth. The goal of this project is to identify PTB-associated epigenetic differences in maternal and fetal samples and to evaluate whether those differences provide insight into the pathophysiology of PTB and development of psychopathology.
Epigenetic Biomarkers of Early Psychotropic Medication Exposure: The use of antipsychotics, antidepressants, and antiepileptics in both pre-pubertal and post-pubertal windows of brain development raise concerns about the functional effects of psychotropic exposure. We recently identified DNA methylation differences in umbilical cord blood of children exposed to prenatal antiepileptics but not antipsychotics or antidepressants. This ongoing project will evaluate the relationship between these epigenetic differences and social, affective and cognitive functioning in early childhood.
Epigenetic and Biobehavioral Determinants of Preterm Birth in Black Women: The proposed research will investigate whether biobehavioral factors linked to PTB influence the regulation and expression of genes to promote PTB in AA women. We will focus our investigation on biobehavioral factors that affect the inflammatory pathway – including poor nutritional status, stress, and reproductive tract infections – as, according to the Institute of Medicine, inflammation predominantly contributes to the excessive rates of PTB experienced by AA women. This research will contribute to a biopsychosocial understanding of within-race risk for PTB, providing insight into important risk and protective factors relevant to AA women who disproportionately experience PTB.
Adult Psychiatric Problems
Genetic and trauma-related risk factors for PTSD: Exposure to early life stress increases the risk of developing posttraumatic stress disorder (PTSD), but not everyone who experiences abuse or trauma develops it. This study explores the genetic and epigenetic correlates of post-traumatic stress disorder in inner-city residents exposed to traumatic events.
Evaluation of PTSD Biomarker Candidates in Fear-Conditioned Mice: We and others have identified DNA methylation differences in the peripheral blood of subjects with PTSD, but it is not clear how these differences relate to epigenetic patterns in the brain or to gene expression. This project evaluates mRNA expression and DNA methylation in the brain and peripheral tissue of fear-conditioned mice.
Nuclear Capture for Cell-Type Specific Epigenetic Analysis of Adipocytes: The goal of this exploratory research grant is to generate monoclonal antibodies to adipocyte-specific nuclear membrane antigens, which can be used to affinity purifying adipocyte nuclei for further molecular genetic and epigenetic analysis. Development of this technology will contribute to research, diagnostics and therapeutics on the numerous diseases descending from obesity and demonstrate the utility of using nuclear membrane antigens to purify nuclei for studies in many other fields of medicine, where cell type specific analyses are needed.
We are very thankful to the following organizations for supporting this work:
|The Brain and Behavior Research Foundation||National Institute of Minority Health and Health Disparities||National Institute of Diabetes and Digestive and Kidney Diseases||The National Institute of Mental Health (NIMH)|